Your Content Goes Here Since the last report we tried to sequence our construct, but every time something went wrong. First the sample was not received by the company and the second time there was a mistake by the analysis. But we do not give up! It seemed that we have achieved [...]

Your Content Goes Here Our goal for this week was clear: we wanted to have green colonies. Since the last weeks cloning process hasn’t worked out, we had to repeat some steps. At first, we had to isolate the Plasmid psb1C3-RFP from our red E.coli TOP10 cells. This worked out perfectly [...]

Your Content Goes Here Lab-Review Weeks 32-35 Within our first iteration of the project the main objective was to generate a single cell biosensor for phosphate detection. At low phosphate levels the utilized phoA promoter is active which leads to the expression of the lacI repressor. By binding of lacI to [...]